Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia
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Data
2019-03-09Autor
Annisa, Annisa
Hafzari, Rini
Setiawati, Tia
Irawan, Budi
Kusmoro, Joko
Metadane
Pokaż pełny rekordStreszczenie
Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on
knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing
five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers.
Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species.
Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.
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