In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae
Oglądaj/ Otwórz
Data
2020-09-14Autor
Kacániová, Miroslava
Terentjeva, Margarita
Žiarovská, Jana
Kowalczewski, Przemysław Łukasz
Metadane
Pokaż pełny rekordStreszczenie
The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to
evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American
foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of
selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’
intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall,
five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species)
and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with
16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the
most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was
assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in
the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and
L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of
AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were
assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had
the highest antimicrobial activity against P. larvae.
Kolekcje
- Artykuły naukowe UPP [92]
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