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dc.contributor.authorOszako, Tomasz
dc.contributor.authorSikora, Katarzyna
dc.contributor.authorBelbahri, Lassaâd
dc.contributor.authorNowakowska, Justyna A.
dc.identifier.otherdoi 10.1515/ffp-2016-0028
dc.description.abstractIn Poland, about 20% of forest nurseries use irrigation water coming from natural superficial reservoirs, presumed to be the first source of infection caused by harmful pathogens belonging to the Oomycota class, especially Phytophthora genus and Pythium genus. The forest nursery is the only place where forest managers can react before pathogens leave it with asymptomatic plants or soil attached to their roots. The aim of this research was detection and identification phytopathogens in water samples. In order to recognise genus Phytophthora or Pythium in water collected from 33 places in five different forest districts in Poland, two DNA-based approaches of identification were applied: (i) the TaqMan probes, and (ii) sequencing of the ITS6/4 region. The genomic DNA was obtained from 17 of 33 investigated water samples. TaqMan probes helped to identify 8 oomycetes present in 17 water samples. Based on ITS rDNA sequencing data, pathogens were identified in 17 cases, and this to the genus level (6 cases) and to the species level (11 cases). In total five Oomycetes species were identified, i.e. 3 Pythium species (Py. citrinum, Py. angustatum, Py. helicoides) and two Phytophthora species (P. lacustris sp. nov. – former taxon Salixsoil, P. gallica sp. nov.).en
dc.publisherThe Forest Sciences and Committee on Forestry Sciences and Wood Technology of the Polish Academy of Sciences; Instytut Badawczy Lesnictwa (Forest Research Institute), Sekocin Stary, Polanden
dc.rightsCreative Commons Uznanie autorstwa na tych samych warunkach 3.0 Polska
dc.subjectwater coursesen
dc.subjectTaqMan probesen
dc.subjectforest nurseriesen
dc.titleMolecular detection of oomycetes species in water coursesen
dc.contributor.organizationBialystok University of Technology, Faculty of Forestry in Hajnówkapl_PL
dc.contributor.organizationForest Research Institute, Department of Forest Protection, Sękocin Starypl_PL
dc.contributor.organizationUniversity of Neuchâtel, Laboratory of Soil Biologypl_PL
dc.contributor.organizationForest Research Institute, Laboratory of Molecular Biology, Sękocin Starypl_PL
dc.description.epersonPrzemysław Szmit

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Creative Commons Uznanie autorstwa na tych samych warunkach 3.0 Polska
Except where otherwise noted, this item's license is described as Creative Commons Uznanie autorstwa na tych samych warunkach 3.0 Polska