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dc.contributor.authorWaga, Jacek
dc.contributor.authorZientarski, Jerzy
dc.contributor.authorSzaleniec, Maciej
dc.contributor.authorObtułowicz, Krystyna
dc.contributor.authorDyga, Wojciech
dc.contributor.authorSkoczowski, Andrzej
dc.date.accessioned2017-03-02T12:21:05Z
dc.date.available2017-03-02T12:21:05Z
dc.date.issued2012
dc.identifier.citationJ. Waga, J. Zientarski, M. Szaleniec, K. Obtułowicz, W. Dyga, A. Skoczowski, "Null Alleles in Gliadin Coding Loci and Wheat Allergenic Properties", Am. J. Plant Sci., 4 (2013) 160-168pl_PL
dc.identifier.issn2158-2742
dc.identifier.urihttps://depot.ceon.pl/handle/123456789/11635
dc.description.abstractWheat gliadin proteins-an important, nutritional component of many food products may also act as allergenic proteins causing various, clinical symptoms of IgE-mediated food allergies. Gliadins are coded by six complex loci on the chromosomes 1A, 1B, 1D, 6A, 6B and 6D of wheat genome. Each of the loci coding from a few to a dozen of polypep-tides may spontaneously mutate to inactive gene variants called null alleles that do not code any proteins at all. The aim of the present work was to find out whether null alleles in some gliadin coding loci may decrease wheat allergenic properties. Six winter wheat genotypes: gliadin deletion lines (GDL) containing null alleles on 1D, 1B and 6B chromo-somes and control lines (CL) containing active gene variants in all gliadin coding loci, were developed using plant breeding methods. Allergenic properties of the six analyzed hybrids were estimated by ELISA using polled sera of five patients allergic to gluten. Estimated immunoreactivity of GDLs was from 6% to 18% lower as compared with CLs. The obtained results evidenced that gliadin null alleles decrease wheat allergenic properties and may be used as parental forms for breeding of hypoallergenic wheat genotypes.pl_PL
dc.description.sponsorshipThe Polish National Center of Science research grant No. N N310/1622/38.pl_PL
dc.publisherScientific Research Publishing (SCIRP)pl_PL
dc.rightsCreative Commons Uznanie autorstwa na tych samych warunkach 3.0 Polska
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/pl/legalcode
dc.subjectplant breedingpl_PL
dc.subjectELISApl_PL
dc.subjectRP-HPLCpl_PL
dc.subjectA-PAGEpl_PL
dc.subjectallergenicitypl_PL
dc.subjectgliadinspl_PL
dc.subjectwheatpl_PL
dc.titleNull Alleles in Gliadin Coding Loci and Wheat Allergenic Propertiespl_PL
dc.typeinfo:eu-repo/semantics/articlepl_PL
dc.contributor.organizationCereals Department, Plant Breeding and Acclimatization Institute, Krakowpl_PL
dc.contributor.organizationDepartment of Clinical and Environmental Allergology, Collegium Medicum, Jagiellonian Universitypl_PL
dc.contributor.organizationJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciencespl_PL
dc.contributor.organizationFranciszek Górski Institute of Plant Physiology, Polish Academy of Sciencespl_PL
dc.description.epersonMaciej Szaleniec
dc.rights.DELETETHISFIELDinfo:eu-repo/semantics/openAccess


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