Expression profile of circulating microRNAs in patients with ischemic heart failure with moderately reduced left ventricular ejection fraction – pilot study

Abstract

Introduction: Heart failure (HF) is a growing global pandemic that affects millions of people around the world. Despite the progress in medicine, diagnosis and treatment of HF remains problematic. Recently, noncoding microribonucleic acids called miRNAs have become very significant in the diagnosis and stratification of HF risk.

Aim: The aim of this study was the attempt to identify the profile of circulating miRNAs specific for ischemic HF with moderately reduced left ventricular ejection fraction (HFmrEF).

Methods and Results: A number of changes in the miRNA profile can characterise patients with ischemic HFmrEF. This is a pilot study before further research on a larger group of patients.

Conclusions: Using the quantitative reverse transcription-polymerase chain reaction (qRT-PCR), serum levels of 84 miRNA were measured and compared between a patient with ischemic HFmrEF and a healthy volunteer. Analysis reveals a down-regulation of let-7f-5p and miR-1-3p, as well as up-regulation of miR-100-5p, miR-10b-5p, miR-125a-5p, miR-140-5p, miR-144-3p, miR-149-5p, miR-15b-5p, miR-183-5p, miR-208b-3p, miR-224-5p, miR-26b-5p, miR-27b-3p, miR-302a-3p, miR-320a, miR-7-5p, miR-99a-5p.