Molecularly imprinted polymer as a synthetic receptor mimic for capacitive impedimetric selective recognition of Escherichia coli K-12
Oglądaj/ Otwórz
Data
2021-10-14Autor
Yasmeen, Nabila
Etienne, Mathieu
Sharma, Piyush Sindhu
El-Kirat-Chate, Sofiane
Brites Helú, Mariela
Kutner, Wlodzimierz
Metadane
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We fabricated an electrochemical molecularly imprinted polymer (MIP) chemosensor for rapid identification and quantification of E. coli strain using 2-aminophenyl boronic acid as the functional monomer. This strain is a modified Gram-negative strain of Escherichia coli bacterium, an ordinary human gut component. The E. coli strongly interacts with a boronic acid because of porous and flexible polymers of the cell wall. The SEM imaging showed that the bacteria template was partially entrapped within the polymeric matrix in a single step. Moreover, this imaging confirmed E. coli K-12 cell template extraction effectiveness. The prepared MIP determined the E. coli K-12 strain up to 2.9 x104 cells mL-1. The
interference study performed in the presence of E. coli variants expressing different surface appendages (type 1 fimbriae or Antigen 43 protein) or Shewanella oneidensis MR1, another Gram-negative bacteria,
demonstrated that the bacterial surface composition notably impacts sensing properties of the bacteria imprinted polymer.