Pro-inflammatory cytokines, but not brain- and extracellular matrix-derived proteins, are increased in the plasma following electrically induced kindling of seizures
Data
2021Autor
Chmielewska, Natalia
Maciejak, Piotr
Osuch, Bartosz
Kursa, Miron Bartosz
Szyndler, Janusz
Metadane
Pokaż pełny rekordStreszczenie
Background The aim of the study was to evaluate the brain-derived proteins, extracellular matrix-derived protein and
cytokines as potential peripheral biomarkers of different susceptibility to seizure development in an animal model of epilepsy
evoked by chronic focal electrical stimulation of the brain.
Methods The plasma levels of IL-1β (interleukin 1β), IL-6 (interleukin 6), UCH-L1 (ubiquitin C-terminal hydrolase 1),
MMP-9 (matrix metalloproteinase 9), and GFAP (glial fibrillary acidic protein) were assessed. The peripheral concentra-
tions of the selected proteins were analyzed according to the status of kindling and seizure severity parameters. In our study,
increased concentrations of plasma IL-1β and IL-6 were observed in rats subjected to hippocampal kindling compared to
sham-operated rats.
Results Animals that developed tonic–clonic seizures after the last stimulation had higher plasma concentrations of IL-1β
and IL-6 than sham-operated rats and rats that did not develop seizure. Elevated levels of IL-1β and IL-6 were observed in
rats that presented more severe seizures after the last five stimulations compared to sham-operated animals. A correlation
between plasma IL-1β and IL-6 concentrations was also found. On the other hand, the plasma levels of the brain-derived
proteins UCH-L1, MMP-9, and GFAP were unaffected by kindling status and seizure severity parameters.
Conclusions The plasma concentrations of IL-1β and IL-6 may have potential utility as peripheral biomarkers of immune
system activation in the course of epilepsy and translational potential for future clinical use. Surprisingly, markers of cell
and nerve ending damage (GFAP, UCH-L1 and MMP-9) may have limited utility.
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