Determination of chlorogenic and gallic acids by UPLC-MS/MS
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Data
2013Autor
Gryszczyńska, Agnieszka
Opala, Bogna
Łowicki, Zdzisław
Krajewska-Patan, Anna
Buchwald, Waldemar
Czerny, Bogusław
Mielcarek, Sebastian
Boroń, Dariusz
Bogacz, Anna
Mrozikiewicz, Przemysław
Metadane
Pokaż pełny rekordStreszczenie
The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.
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