Phototoxicity of protoporphyrin IX, diarginine diprotoporphyrinate and N,N-diphenylalanyl protoporphyrin toward human fibroblasts and keratinocytes in vitro: effect of 5-methoxypsoralen
dc.contributor.author | Bugaj, Andrzej | |
dc.contributor.author | Morliere, Patrice | |
dc.contributor.author | Santus, Rene | |
dc.contributor.author | Haigle, Josiane | |
dc.contributor.author | Dyderski, Stanisław | |
dc.date.accessioned | 2015-03-16T21:36:14Z | |
dc.date.available | 2015-03-16T21:36:14Z | |
dc.date.issued | 2004-09-07 | |
dc.identifier.uri | https://depot.ceon.pl/handle/123456789/6486 | |
dc.description.abstract | The phototoxicity of two new porphyrin photosensitizers, diarginine diprotoporphyrinate (PP(Arg)2) and N,N-diphenylalanyl protoporphyrin (PP(Phe)2), and the synergistic effect of 5-methoxyposralen (5-MOP) have been studied in comparison with that of protoporphyrin IX (PPIX). Under ultraviolet-A (UV-A) irradiation (lambda = 365 nm), the phototoxicity of the porphyrins toward cultured human fibroblasts and keratinocytes decreases in the order: PPIX > PP(Arg)2 > PP(Phe)2. A synergistic effect of 5-MOP on the phototoxicity of PPIX, PP(Arg)2 and PP(Phe)2 has been observed. The combination of PPIX, PP(Arg)2 and PP(Phe)2 with 0.1–0.5 micromole/l 5-MOP significantly potentiates the phototoxicity of the three porphyrins. The most effective potentiation was observed with the water-soluble PP(Arg)2 and 5-MOP concentrations lower than 0.75 micromole/l. Above this 5-MOP concentration this potentiation is abolished. The intracellular concentration of PPIX and PP(Phe)2 is independent of the presence of 5-MOP. On the other hand, the intracellular content of PP(Arg)2 is decreased in a concentration-dependent manner by the psoralen. Illumination with red light, not absorbed by 5-MOP, leads to a weak potentiation of the PP(Arg)2 phototoxic effect in the presence of 5-MOP, suggesting that dark interaction of 5- MOP with cell membranes aggravated by porphyrin photosensitization is involved in the observed phenomena. The results are tentatively explained by differences in hydrophobicity and molecular structures of the examined photosensitizers. PPIX, which is barely soluble in water, | pl_PL |
dc.language.iso | en | pl_PL |
dc.publisher | American Society for Photobiology | pl_PL |
dc.rights | Dozwolony użytek | |
dc.subject | phototoxicity | pl_PL |
dc.subject | keratinocytes | pl_PL |
dc.subject | fibroblasts | pl_PL |
dc.subject | 5-methoxypsoralen | pl_PL |
dc.subject | diarginine protoporphyrinate | pl_PL |
dc.subject | sodium salt of protoporphyrin IX | pl_PL |
dc.subject | diphenylalanyl protoporphyrin | pl_PL |
dc.title | Phototoxicity of protoporphyrin IX, diarginine diprotoporphyrinate and N,N-diphenylalanyl protoporphyrin toward human fibroblasts and keratinocytes in vitro: effect of 5-methoxypsoralen | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.contributor.organization | Department of Clinical Pharmacy and of Biopharmacy, Karol Marcinkowski University of Medical Sciences, Poznan | pl_PL |
dc.contributor.organization | Departement RDDM: Equipe de Photobiologie, Museum National d’Histoire Naturelle, Paris, France | pl_PL |
dc.contributor.organization | INSERM U.532, Institut de Recherche sur la Peau, Hopital Saint-Louis, Paris, France | pl_PL |
dc.description.eperson | Andrzej M. Bugaj |
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