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dc.contributor.authorBugaj, Andrzej
dc.contributor.authorMorliere, Patrice
dc.contributor.authorSantus, Rene
dc.contributor.authorHaigle, Josiane
dc.contributor.authorDyderski, Stanisław
dc.date.accessioned2015-03-16T21:36:14Z
dc.date.available2015-03-16T21:36:14Z
dc.date.issued2004-09-07
dc.identifier.urihttps://depot.ceon.pl/handle/123456789/6486
dc.description.abstractThe phototoxicity of two new porphyrin photosensitizers, diarginine diprotoporphyrinate (PP(Arg)2) and N,N-diphenylalanyl protoporphyrin (PP(Phe)2), and the synergistic effect of 5-methoxyposralen (5-MOP) have been studied in comparison with that of protoporphyrin IX (PPIX). Under ultraviolet-A (UV-A) irradiation (lambda = 365 nm), the phototoxicity of the porphyrins toward cultured human fibroblasts and keratinocytes decreases in the order: PPIX > PP(Arg)2 > PP(Phe)2. A synergistic effect of 5-MOP on the phototoxicity of PPIX, PP(Arg)2 and PP(Phe)2 has been observed. The combination of PPIX, PP(Arg)2 and PP(Phe)2 with 0.1–0.5 micromole/l 5-MOP significantly potentiates the phototoxicity of the three porphyrins. The most effective potentiation was observed with the water-soluble PP(Arg)2 and 5-MOP concentrations lower than 0.75 micromole/l. Above this 5-MOP concentration this potentiation is abolished. The intracellular concentration of PPIX and PP(Phe)2 is independent of the presence of 5-MOP. On the other hand, the intracellular content of PP(Arg)2 is decreased in a concentration-dependent manner by the psoralen. Illumination with red light, not absorbed by 5-MOP, leads to a weak potentiation of the PP(Arg)2 phototoxic effect in the presence of 5-MOP, suggesting that dark interaction of 5- MOP with cell membranes aggravated by porphyrin photosensitization is involved in the observed phenomena. The results are tentatively explained by differences in hydrophobicity and molecular structures of the examined photosensitizers. PPIX, which is barely soluble in water,pl_PL
dc.language.isoenpl_PL
dc.publisherAmerican Society for Photobiologypl_PL
dc.rightsDozwolony użytek
dc.subjectphototoxicitypl_PL
dc.subjectkeratinocytespl_PL
dc.subjectfibroblastspl_PL
dc.subject5-methoxypsoralenpl_PL
dc.subjectdiarginine protoporphyrinatepl_PL
dc.subjectsodium salt of protoporphyrin IXpl_PL
dc.subjectdiphenylalanyl protoporphyrinpl_PL
dc.titlePhototoxicity of protoporphyrin IX, diarginine diprotoporphyrinate and N,N-diphenylalanyl protoporphyrin toward human fibroblasts and keratinocytes in vitro: effect of 5-methoxypsoralenpl_PL
dc.typeinfo:eu-repo/semantics/articlepl_PL
dc.contributor.organizationDepartment of Clinical Pharmacy and of Biopharmacy, Karol Marcinkowski University of Medical Sciences, Poznanpl_PL
dc.contributor.organizationDepartement RDDM: Equipe de Photobiologie, Museum National d’Histoire Naturelle, Paris, Francepl_PL
dc.contributor.organizationINSERM U.532, Institut de Recherche sur la Peau, Hopital Saint-Louis, Paris, Francepl_PL
dc.description.epersonAndrzej M. Bugaj


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