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dc.contributor.authorSzyp-Borowska, Iwona
dc.contributor.authorBanha, Catarina
dc.contributor.authorWojda, Tomasz
dc.contributor.authorSzczygieł, Krystyna
dc.identifier.otherDOI: 10.1515/ffp-2016-0002
dc.description.abstractThe study on the in vitro micropropagation of Robinia pseudoacacia L. shows the possibility of the rapid increase of the vegetative progeny using organogenesis. The culture system consists of sequential use of three media, namely, the initiation medium (MS medium supplemented with 0.6 mg · l-1 6-BA (6-benzylamino-purine), 0.1 mg · l-1 NAA (naphthalene acetic acid), proliferation medium (1/2 MS medium added with 1 mg · l-1 6-BA,) and root-induction medium (1/2 MS medium fortified with 0.2 mg · l-1 NAA). The resulting micropropagated plants, fully acclimatised to an in vivo environment, did not show any visually detectable morphological variation. In addition, we investigated the genetic stability (vegetative progeny to the donor plant) of a sample of 30 plants randomly taken from ca. 1067 micropropagated plants, by using three microsatellites loci Rops 15, Rops 16, Rops 18. Our results suggest that DNA sequence variations may occur in micropropagated plants.pl_PL
dc.publisherInstytut Badawczy Lesnictwa (Forest Research Institute), Komitet Nauk Lesnych PAN (The Committee on Forestry Sciences of the Polish Academy of Sciences)pl_PL
dc.rightsCreative Commons Uznanie autorstwa na tych samych warunkach 3.0 Polska
dc.subjecttissue culturespl_PL
dc.subjectgenetic stabilitypl_PL
dc.subjectblack locustpl_PL
dc.titleMicropropagation of black locust (Robinia pseudoacacia L.)and genetic stability of long term cultivated plantspl_PL
dc.contributor.organizationForest Research Institute, Department of Silviculture and Genetics of Forest Trees, Sękocin Starypl_PL
dc.contributor.organizationInstituto Politécnico de Coimbra, Escola Superior Agrária, Portugalpl_PL
dc.description.epersonPrzemysław Szmit

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Creative Commons Uznanie autorstwa na tych samych warunkach 3.0 Polska
Except where otherwise noted, this item's license is described as Creative Commons Uznanie autorstwa na tych samych warunkach 3.0 Polska