Micropropagation and in vitro rooting of Robinia pseudoacacia L. recalcitrant genotypes

Thumbnail Image
DOI-10.2478-ffp-2020-0002.pdf (2.58 MB)
Authors
Szyp-Borowska, Iwona
Ukalska, Joanna
Wojda, Tomasz
Sułkowska, Małgorzata
Klisz, Marcin
Date
2020-11-04
Journal Title
Journal ISSN
Volume Title
Publisher
The Committee on Forestry Sciences and Wood Technology of the Polish Academy of Sciences and the Forest Research Institute in Sekocin Stary
License
Creative Commons License
Creative Commons Attribution-NoDerivs 3.0 Poland License
Abstract
In forest production, there is an emerging tendency towards the planting of fast-growing trees as attractive, renewable energy sources. Hence, efforts were made to develop a method of micropropagation by organogenesis of seven clones of black locust (Robinia pseudoacacia L.) that are resistant to propagation by traditional vegetative methods, as well as one plus tree (no. 9755) at the age of 60, to see if the age of the mother plant is a limitation in the micropropagation of black locust trees. Overall results suggest that Murashige and Skoog medium supplemented with 30 g l−1 sucrose, 0.6 mg l−1 6-benzylaminopurine (BAP) and 0.1 mg l−1 naphthalene acetic acid (NAA) is better for the propagation of each genotype of R. pseudoacacia than Woody Plant Medium with the same growth regulators, and the age of the donor plant does not affect the organogenic potential. Recalcitrance to adventitious rooting from adventitious shoot formation is a major limitation for the clonal micropropagation of forest trees. Our results showed that although the roots were also formed spontaneously in the growth medium without growth hormones for the tested black locust clones, the application of auxin increased the total root length compared to that in the medium with active carbon and control. A significant effect of the additives of hormone and sucrose on the total root length was found. Increasing the sucrose concentration stimulated the induction of roots in each of the tested concentrations (5, 10, 15 or 20 g l−1). Additionally, the change in sugar dose in the rooting medium caused significant differences in total root length.
Description
Keywords
black locust    in vitro cultures    medium effect
Citation
Szyp-Borowska, I., Ukalska, J., Wojda, T., Sułkowska, M., & Klisz, M. (2020). Micropropagation and in vitro rooting of Robinia pseudoacacia L. recalcitrant genotypes, Folia Forestalia Polonica, 62(1), 13-21. doi: https://doi.org/10.2478/ffp-2020-0002
URI
https://depot.ceon.pl/handle/123456789/18825
Belongs to collection
Artykuły / Articles